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KMID : 0352819920080010057
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Kosin Medical Journal
1992 Volume.8 No. 1 p.57 ~ p.62
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Comparative Study on the Specificity and the Sensitivity of Immunoserlolgic Trematodes
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Abstract
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To study the specificity and the sensitivity of immunodiagnostic tests for 3 closely related species of trematodes, Clonorcbis sinensis. Paragonimus westermani, Fasciola bepatica were used as antigens. Antisera of 13 clonorchiasis patients, 5
paragonimiasis patients, 1 fascioliasis patients, and 11 normal adults human sera were applied.
Clonorchis antigen and antibody reaction in double-diffusion was weak, and only 23% among patients was positive. But paragonimiasis antisera-antigen reaction was very clear and 4 of 5 was positive. Clonorchiasis antisera-antisera was 100%
positive in micro-ELISA, but clonorchis antigen, paragonimus antigen, fasciola antigen-fascioliasis antisera reation was also 100% positive.
In dot-ELISA, clonorchis antigen-antisera was 11 patients positive among 13 patients, but reaction of clonorchis antigen-paragonimus antisera was also 3 positive among 5 patients. Paragonimus antigencolnorchis antibody never reacted.
but
1 of
5 normal human sera reacted with clonorchis and paragonimus antigen. And fasciola antigen made cross-reaction with paragonimiasis patient sera and 10 of 13 clonorchiasis patient sera.
In this experiments, although there was no cross-reactivity in double-diffusion, sensitivity was low. Micro and Dot-ELISA had high sensitivity, but specificity was low. And it was considered that the apprepriate mixture of 3 kinds of test
will
produce the good results.
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